Protection of abasic sites during DNA replication by a stable thiazolidine protein-DNA cross-link
نویسندگان
چکیده
منابع مشابه
Translesion replication of abasic sites in DNA
Abasic sites (AP sites) arise very frequently in DNA and represent a common intermediate in numerous DNA repair systems. However, an unrepaired AP site poses a serious problem to the advancement of DNA polymerases (Pols) because the modified base has lost its coding capacity. Nevertheless, many Pols, either replicatives or specialized, can replicate AP sites, at least in vitro, with variable ef...
متن کاملInterstrand DNA–DNA Cross-Link Formation Between Adenine Residues and Abasic Sites in Duplex DNA
The loss of a coding nucleobase from the structure of DNA is a common event that generates an abasic (Ap) site (1). Ap sites exist as an equilibrating mixture of a cyclic hemiacetal and a ring-opened aldehyde. Aldehydes are electrophilic functional groups that can form covalent adducts with nucleophilic sites in DNA. Thus, Ap sites present a potentially reactive aldehyde as part of the internal...
متن کاملInterstrand cross-link of DNA by covalently linking a pair of abasic sites.
A pair of apurinic/apyrimidinic sites formed in DNA has been covalently connected with bis(aminooxy) derivatives. The efficacy of the interstrand cross-link is associated with the structural tethering of two aminooxy groups. The interstrand cross-link constructed stable DNA scaffolds for enzyme alignment.
متن کاملInterstrand cross-links generated by abasic sites in duplex DNA.
Abasic sites (1, Scheme 1), which are generated by hydrolysis of the glycosidic bonds connecting the heterocyclic nucleobases to the deoxyribose backbone, represent the most common type of damage suffered by genomic DNA.1 Spontaneous hydrolysis yields approximately 10,000 abasic sites per cell per day. In addition, exposure of DNA to radiation, mutagens, and anticancer drugs can lead to the gen...
متن کاملCharacterization of Interstrand DNA-DNA Cross-Links Derived from Abasic Sites Using Bacteriophage ϕ29 DNA Polymerase.
Interstrand cross-links in cellular DNA are highly deleterious lesions that block transcription and replication. We recently characterized two new structural types of interstrand cross-links derived from the reaction of abasic (Ap) sites with either guanine or adenine residues in duplex DNA. Interestingly, these Ap-derived cross-links are forged by chemically reversible processes, in which the ...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Nature Structural & Molecular Biology
سال: 2019
ISSN: 1545-9993,1545-9985
DOI: 10.1038/s41594-019-0255-5